Goat anti-58K Golgi protein(N-Term)/FTCD Antibody

• Catalog Number: orb18848
• Category: Antibodies
• Description: Goat polyclonal to 58K Golgi protein
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse, Porcine
• Dilution range: ELISA: 1:64000, WB: 0.05-0.2 μg/ml
• Conjugation: Unconjugated
• MW: 58.9; 61.3
• Target: 58K Golgi protein(N-Term)/FTCD
• Entrez: 10841
• Protein Sequence: SQLVECVPNFSEGKNQ
• RRID: AB_10752684
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti FTCD antibody, anti LCHC1 antibody, anti form
• Note: For research use only
• Application notes: Variants (NP_006648.1; NP_996848.1) encode the same protein.ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration, 3-5ug/ml.WB: A 55-60kDa band observed in Human Liver lysate (calculated MW of 58.9kDa according to NP_006648. Recommended concentration: 0.03-0.5 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18848 (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18848 (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.