Goat 53BP1 Antibody

SKU: orb1523957

Description

53BP1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 375 and 425
• Target: 53BP1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

53BP1; p202; p53-binding protein 1; p53BP1; TDRD30; TP53-binding protein 1; tumor protein 53-binding protein, 1; tumor suppressor p53-binding protein 1

Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-53BP1 antibody orb1523957 used for IP at 6 µg/mg lysate.

Detection of human 53BP1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-53BP1 (Cat. No. orb1523957) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

53BP1

UniProt

Q12888

RefSeq (Protein):NP_005648.1