4E-T/EIF4ENIF1 Antibody

SKU: orb18625

Description

Goat polyclonal antibody to EIF4ENIF1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.25-0.5 μg/ml, IHC-P: 5-10µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10ug/ml.WB: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5 μg/ml

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: 4E-T / EIF4ENIF1
• Protein Sequence: AKVISVDELEYRQ
• Molecular Weight: 108; 88.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti EIF4ENIF1 antibody, anti 4E-T antibody, anti Clast4 antibody, anti FLJ21601 antibody, anti 2610509L04Rik antibody, anti eukaryotic translation initiation factor 4E nuclear import factor 1 antibody, anti eIF4E-transporter antibody, anti FLJ26551 antibody, anti OTTHUMP00000063276 antibody

orb18625 staining (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18625 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

4E-T / EIF4ENIF1

NCBI Gene ID

56478