Goat anti-4E-T / EIF4ENIF1 Antibody

• Catalog Number: orb18625
• Category: Antibodies
• Description: Goat polyclonal antibody to EIF4ENIF1
• Target: 4E-T / EIF4ENIF1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Protein Sequence: AKVISVDELEYRQ
• RRID: AB_10755581
• MW: 108; 88.2
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.25-0.5 μg/ml, IHC-P: 5-10µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10ug/ml.WB: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5 μg/ml
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti EIF4ENIF1 antibody, anti 4E-T antibody, anti
• Note: For research use only
• Entrez: 56478

orb18625 staining (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18625 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.