14-3-3 sigma/Stratifin Antibody

SKU: orb19148

Description

Goat polyclonal antibody to SFN

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.03-0.1 μg/ml, IHC-P: 2-4µg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer . Recommended concentration, 2-3ug/ml.WB: Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: 14-3-3 sigma / Stratifin
• Molecular Weight: 27.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

SFN, 14-3-3sigma, stratifin, HGNC:10773, YWHAS

Immunohistochemical analysis of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. with SFN antibody.

Western blot analysis of staining of Human Skin lysate (35µg protein in RIPA buffer) using SFN antibody.

Quick Database Links

Gene Symbol

14-3-3 sigma / Stratifin

NCBI Gene ID

2810