Goat AKT2 Antibody

SKU: orb2899332

Description

Goat AKT2 Antibody

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:1000. Immunofluorescence: Strong expression of the protein seen in the cytoplasm, vesicles and plasma membranes of A549 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Host: Goat
• Immunogen: Peptide with sequence C-YKERPEAPDQTLP, from the internal region of the protein sequence according to NP_001617.1.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AKT2, v-akt murine thymoma viral oncogene homolog 2, PKBBETA, PRKBB, RAC-BETA, Murine thymoma viral (v-akt) homolog-2, rac protein kinase beta

Immunofluorescence analysis of paraformaldehyde fixed A549. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic, vesicles and membrane staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

RefSeq (Protein):NP_001617.1