Goat ABCG1 Antibody

SKU: orb2899330

Description

Goat ABCG1 Antibody

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx. 75kDa band observed in lysates of cell lines HepG2, U937, THP-1, Jurkat and RAW264.7 (calculated MW of 74.2kDa according to Human NP_058198.2 and 74.1kDa according to Mouse NP_033723.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml. Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml.
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat

Key Properties

• Host: Goat
• Immunogen: Peptide with sequence C-YFVLRYKIRAER, from the C Terminus of the protein sequence according to NP_058198.2; NP_997057.1; NP_004906.3; NP_997510.1; NP_997511.1; NP_997512.1.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

ABCG1, ATP-binding cassette, sub-family G (WHITE), member 1, ABC8, MGC34313, WHITE1, ABC transporter 8, ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, ATP-binding cassette transporter member 1 of subfamily G, homolog of Drosophila white, white protein homolog (ATP-binding cassette transporter 8)

Primary incubation 1 hour at room temperature. Images A, B, C: HepG2, U937, THP1 cell lysate at primary Ab concentration 2 ug/ml. Images D, E: Jurkat, RAW264.7 cell lysate at primary Ab concentration 1 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

5 µg/ml staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Quick Database Links

RefSeq (Protein):NP_997511.1, NP_058198.2, NP_004906.3, NP_997512.1, NP_997057.1, NP_997510.1