GNIP Antibody

• Catalog Number: orb1247437
• Category: Antibodies
• Description: GNIP Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QAPAGPPWDITEA
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TRIM7
• UniProt ID: Q9C029
• NCBI: NP_203128.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TRIM7, GNIP1, RNF90, tripartite motif-containing 7
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 25-30kDa band observed in Human Skeletal Muscle lysate (predicted MW of 25kDa according to NP_203128). Recommended for use at 1ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows pixulate staining in the fibres. Recommended concentration, 3-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247437 (1 ug/ml) staining of Human Skeletal Muscle lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247437 (3.8 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.