TRIM7 Antibody

SKU: orb1247437

Description

TRIM7 Antibody

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 25-30kDa band observed in Human Skeletal Muscle lysate (predicted MW of 25kDa according to NP_203128). Recommended for use at 1µg/mL.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows pixulate staining in the fibres. Recommended concentration, 3-6µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QAPAGPPWDITEA
• Target: TRIM7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TRIM7, GNIP1, RNF90, tripartite motif-containing 7, tripartite motif protein TRIM7, glycogenin-interacting protein 1, GNIP, glycogenin-interacting protein

orb1247437 (1 ug/ml) staining of Human Skeletal Muscle lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247437 (3.8 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

TRIM7

UniProt

Q9C029

RefSeq (Protein):NP_203128.1