GNB2L1 Antibody

• Catalog Number: orb1249294
• Category: Antibodies
• Description: GNB2L1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: NTLGVCKYTVQDESH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Lymph Nodes, in Mouse and Rat Spleen lysates and in lysates of many cell lines (calculated MW of 35.1kDa according to NP_006089.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GNB2L1
• UniProt ID: P63244
• NCBI: NP_006089.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GNB2L1, guanine nucleotide binding protein (G prot
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Lymph Nodes, in Mouse and Rat Spleen lysates and in lysates of many cell lines (calculated MW of 35.1kDa according to NP_006089.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249294 (0.1 ug/ml) staining of Human Lymph Node (A), Mouse Spleen (B) and Rat Spleen (C) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249294 (0.1 ug/ml) staining of NIH3T3 (A), HeK293 (B), HeLa (C, A431 (D), A549 (E), MCF7 (F), Jurkat (G), K562 (H) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249294 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.