GNB2L1 Antibody

• Catalog Number: orb1249294
• Category: Antibodies
• Description: GNB2L1 Antibody
• Target: GNB2L1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: NTLGVCKYTVQDESH
• UniProt ID: P63244
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Lymph Nodes, in Mouse and Rat Spleen lysates and in lysates of many cell lines (calculated MW of 35.1kDa according to NP_006089.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GNB2L1, guanine nucleotide binding protein (G prot
• Note: For research use only
• NCBI: NP_006089.1

orb1249294 (0.1 ug/ml) staining of Human Lymph Node (A), Mouse Spleen (B) and Rat Spleen (C) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249294 (0.1 ug/ml) staining of NIH3T3 (A), HeK293 (B), HeLa (C, A431 (D), A549 (E), MCF7 (F), Jurkat (G), K562 (H) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249294 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.