GNAQ Antibody

• Catalog Number: orb1249253
• Category: Antibodies
• Description: GNAQ Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-NDLDRVADPAYLPT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in Human, Mouse and Rat Testis lysates, in Pig Colon and Spleen lysates and in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 42.1kDa according to Human NP_002063.2 and to Rat NP_112298.1 ). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GNAQ
• UniProt ID: P50148
• NCBI: NP_002063.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GNAQ, guanine nucleotide binding protein (G protei
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in Human, Mouse and Rat Testis lysates, in Pig Colon and Spleen lysates and in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 42.1kDa according to Human NP_002063.2 and to Rat NP_112298.1 ). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249253 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Testis lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249253 (0.1 ug/ml) staining of Peripheral Blood Lymphocytes lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249253 (0.1 ug/ml) staining of Pig Colon (A) and Pig Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249253 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.