GNAQ Antibody

SKU: orb1249253

Description

GNAQ Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in Human, Mouse and Rat Testis lysates, in Pig Colon and Spleen lysates and in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 42.1kDa according to Human NP_002063.2 and to Rat NP_112298.1 ). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NDLDRVADPAYLPT
• Target: GNAQ
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

GNAQ, guanine nucleotide binding protein (G protein), q polypeptide, G-ALPHA-q, GAQ

orb1249253 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Testis lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249253 (0.1 ug/ml) staining of Peripheral Blood Lymphocytes lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249253 (0.1 ug/ml) staining of Pig Colon (A) and Pig Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249253 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

GNAQ

UniProt

P50148

RefSeq (Protein):NP_002063.2