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Item 1 of 6
Item 1 of 6
GNA13 Antibody, KO Validated
Catalog Number: orb1274443
| Catalog Number | orb1274443 |
|---|---|
| Category | Antibodies |
| Description | GNA13 Antibody, KO Validated |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant protein of human GNA13 |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 44kDa |
| Target | GNA13 |
| UniProt ID | Q14344 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | Guanine nucleotide-binding protein subunit alpha-1 Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using GNA13 antibody (orb1274443) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and GNA13 knockout (KO) HeLa cells, using GNA13 antibody (orb1274443) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using GNA13 antibody (orb1274443) (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GNA13 antibody (orb1274443) (40x lens).
Immunofluorescence analysis of C6 cells using GNA13 Polyclonal Antibody (orb1274443) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GNA13 Polyclonal Antibody (orb1274443) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
