GNA11 Antibody (Center)

• Catalog Number: orb1926465
• Category: Antibodies
• Description: GNA11 Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P29992
• MW: 42123 Da
• Tested applications: IF, WB
• Dilution range: WB - 1:1000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: GA11
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only

All lanes: Anti-GNA11 Antibody (Center) at 1:2000 dilution. Lane 1: human testis lysates. Lane 2: Hela whole cell lysates. Lane 3: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling GNA11 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and membrane staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).