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GNA11 Antibody (Center)
Catalog Number: orb1926465
| Catalog Number | orb1926465 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This GNA11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 115-146 amino acids from the Central region of human GNA11. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | P29992 |
| MW | 42123 Da |
| Tested applications | IF, WB |
| Dilution range | IF: 1:25, WB: 1:2000 |
| Clone Number | RB52469 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Guanine nucleotide-binding protein subunit alpha-1 Read more... |
| Note | For research use only |
All lanes: Anti-GNA11 Antibody (Center) at 1:2000 dilution. Lane 1: human testis lysates. Lane 2: Hela whole cell lysates. Lane 3: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling GNA11 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and membrane staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).
