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CSF2 Antibody

SKU: orb750689

Description

CSF2 antibody

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
ReactivityHuman
Application Notes
This antiserum against Human GM-CSF has been tested for use in immunoblotting. Reactivity is also expected in neutralizations, radioimmunoassay, ELISA and immunohistochemistry. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeAntiserum
ImmunogenThis whole rabbit serum was prepared by repeated immunizations with full length recombinant human GM-CSF.
TargetCSF2
PurityThis antiserum has been heated to 56°C for 30 minutes. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. In ELISA and other immunoreactive assays, this antiserum will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesNone
Concentration85 mg/mL
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

Molgramostin antibody, Pluripoietin Alpha antibody, Sargramostim antibody, Colony stimulating factor antibody, CSF 2 antibody, CSF Alpha antibody, Burst Promoting Activity antibody, rabbit anti-GM-CSF, rabbit anti-granulocyte-macrophage colony-stimulating factor antibody

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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