GM-CSF Antibody

• Catalog Number: orb345215
• Category: Antibodies
• Description: GM-CSF antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse GM-CSF protein.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: This purified antibody has been purified by a multi-step process which includes delipidation, salt fractionation, and heat treatment to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of mouse GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: Unconjugated
• UniProt ID: P01587
• NCBI: NP_034099.2
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: rabbit anti-GM-CSF antibody, rabbit anti-Granulocy
• Note: For research use only
• Application notes: This purified antibody has been tested in western blot and is suitable for use in neutralizations and ELISA. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. By western blot a band approximately 14.3 kDa in size corresponding to mouse GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's anti-Mouse GM-CSF antibody shows detection of a band ~14 kDa in size corresponding to recombinant mouse GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using DyLight™649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20000 in blocking buffer (p/n orb348637).