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Csf2 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mGM-CSF. Anti-Murine GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix. |
| Target | Csf2 |
| Purification | GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−GMCSF antibody [orb6090]
ICC, IF, IHC-P, WB
Guinea pig, Human, Porcine, Rat
Rabbit
Polyclonal
Unconjugated
100 μgHuman Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit [orb774893]
Human
15.63-1000 pg/mL
6.5 pg/mL
96 T, 48 TMouse Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit [orb774894]
Mouse
15.63-1000 pg/mL
0.57 pg/mL
96 T, 48 TRat Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit [orb774895]
Rat
7.82-500 pg/mL
3 pg/mL
48 T, 96 THuman Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit [orb778165]
Human
0.32-20 ng/mL
0.113 ng/mL
48 T, 96 T

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To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine GM-CSF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.

To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
Csf2 Antibody (orb1671457)
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