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Glypican 3 (GPC3) Antibody (Center)
Catalog Number: orb1930078
| Catalog Number | orb1930078 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB5036 |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: Tested, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 65563 Da |
| Target | This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-365 amino acids from the Central region of human Glypican 3 (GPC3). |
| UniProt ID | P51654 |
| NCBI | NP_001158089.1, NP_004475.1, NP_001158091.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorecence staining of anti-GPC3 Pab on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1: 50. Original magnification 1:400.
Anti-GPC3 Antibody (C349) at 1:1000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
