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Catalog Number | orb1930077 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB5038 |
Tested applications | IF, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 65563 Da |
Target | This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529-560 amino acids from the C-terminal region of human Glypican 3 (GPC3). |
UniProt ID | P51654 |
NCBI | NP_001158089.1, NP_004475.1, NP_001158091.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorecence staining of anti-GPC3 Pab on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1: 50. Original magnification 1:400.
GPC3 Antibody (P544) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).
GPC3 Antibody (P544) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).