Glycoprotein 2 Antibody / GP2 / ZAP75

SKU: orb639687

Description

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Glycoprotein 2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2c, kappa
• Clone No.: GP2/3416
• Immunogen: A recombinant human partial protein (amino acids 35-179) was used as the immunogen for this Glycoprotein 2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human pancreas with Glycoprotein 2 antibody (clone GP2/3416). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE human pancreas with Glycoprotein 2 antibody (clone GP2/3416). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human pancreas lysate with Glycoprotein 2 antibody (clone GP2/3416).

SDS-PAGE analysis of purified, BSA-free Glycoprotein 2 antibody (clone GP2/3416) as confirmation of integrity and purity.

Quick Database Links

UniProt

P55259