NMDAR2A NMDA Antibody (FITC)

SKU: orb150321

Description

Mouse monoclonal antibody against GRIN2A conjugated to FITC

Research Area

Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	ICC, IF, IHC, WB
Dilution Range	WB (1:1000)
Reactivity	Human, Mouse, Rat
Application Notes	1 µg/ml of SMC-429 was sufficient for detection o GluN2A in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2A
Clone No.	N327/95
Immunogen	Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A
Target	NMDAR2A NMDA
Molecular Weight	170kDa
Purification	Protein G Purified
Conjugation	FITC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NMDA 2A, NMDAR2A, NMDAR 2A, NMDA Receptor 2A, Glutamate Receptor, GRIN2A, Glutamate [NMDA] Receptor subunit epsilon-1, Glutamate receptor ionotropic N methyl D aspartate 2A, HNR2A, N methyl D aspartate receptor channel, subunit epsilon 1, N Methyl D Aspartate Receptor Subtype 2A, N methyl D aspartate receptor subunit 2A, NMDA receptor subtype 2A, NMDA Receptor Type 2A, OTTHUMP00000160135, OTTHUMP00000174531

Quality Guarantee

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone N327/95. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NR2A Antibody (D) Composite.

Quick Database Links

Gene Symbol

NMDAR2A NMDA

UniProt

Q00959

NCBI Gene ID

24409

RefSeq (Protein):NP_036705.3

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_036705.3

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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