GluN1 Antibody (HRP)

SKU: orb149999

Description

Mouse monoclonal to GluN1-NR1 (HRP). The NMDA receptor (NMDAR), a glutamate receptor, is the predominant molecular device for controlling synaptic plasticity and memory function. The NMDA receptor forms a heterotetramer between two NR1 and two NR2 subunits (the subunits are also called glutamate-binding NMDA receptor subunits or GluN for short); two obligatory NR1 subunits and two regionally localized NR2 subunits. A related gene family of NR3 A and B subunits have an inhibitory effect on receptor activity. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits..

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-410 was sufficient for detection of NR1 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N308/48
• Immunogen: Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1
• Target: GluN1
• Molecular Weight: 105kDa
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GRIN1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, Glutamate receptor ionotropic N methyl D aspartate 1, MRD8, Methyl-D-aspartate receptor subunit zeta-1, N methyl D aspartate receptor, N methyl D aspartate receptor channel subunit zeta 1, N methyl D aspartate receptor subunit NR1, NMD-R1, NMDA 1, NMDA R1, NMDA Receptor 1, NMDA1, NMDAR, NMDAR1, NMDZ1_HUMAN, NR1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN1/NR1 Monoclonal Antibody, Clone N308/48. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN1/NR1 Antibody (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone N308/48. Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

GluN1

UniProt

P35439

NCBI Gene ID

24408

RefSeq (Protein):NP_058706.1