Glucose Oxidase Antibody Biotin Conjugated

SKU: orb344933

Description

Glucose Oxidase antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: User Optimized, WB: User Optimized
• Reactivity: Fungi
• Application Notes:
  Anti-GLUCOSE OXIDASE Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 µg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Glucose Oxidase [A.niger]
• Purity: Anti-GLUCOSE OXIDASE Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [A.niger]. Cross reactivity against Glucose Oxidase from other sources may occur but have not been specifically determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Glucose Oxidase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Western Blot of Rabbit anti-Glucose Oxidase antibody Biotin Conjugated. Lane 1: Glucose Oxidase. Load: 50 ng per lane. Primary antibody: Glucose Oxidase antibody Biotin conjugated at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) for 30 min at RT. Predicted/Observed size: 66 kDa, 66 kDa for Glucose Oxidase.

Quick Database Links

UniProt

P13006

RefSeq:P13006.1