GluR1 Antibody (RPE)

SKU: orb150512

Description

Mouse monoclonal to GluA1 (RPE). Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1, human glutamate receptor type 1, is an integral membrane protein that is widely expressed in the human brain. The postsynaptic actions of glutamic acid are mediated by a variety of receptors that are named according to their selective agonists. GluR1 is known to bind a kainate subtype of agonist. It has been found that malfunctioning of the glutamatergic system may result in certain brain disorders and neurodegeneration..

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-440 was sufficient for detection of GluA1 in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N355/1
• Immunogen: Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1
• Target: GluR1
• Molecular Weight: 100kDa
• Purification: Protein G Purified
• Conjugation: RPE

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2900051M01Rik, AI853806, AMPA 1, AMPA selective glutamate receptor 1, GLUH 1, GLUH1, GLUR1, GLURA, Glr-1, Glr1, GluA1, GluR K1, GluR-A, GluR1, GluRA, GluRK1, Glur-1, Glur1, Glutamate receptor 1, Glutamate receptor ionotropic, Glutamate receptor ionotropic AMPA 1, Gria 1, HBGR1, HIPA, MGC13325, OTTHUMP00000160643, OTTHUMP00000165781, OTTHUMP00000224241, OTTHUMP00000224242, OTTHUMP00000224243, gluR-

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Monoclonal Antibody, Clone N355/1. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluA1/GluR1 Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluA1/GluR1 Antibody (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluA1-GluR1 protein using Mouse Anti-GluA1-GluR1 Monoclonal Antibody, Clone N355/1. Load: 10 μg. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluA1-GluR1 Monoclonal Antibody at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.

Quick Database Links

Gene Symbol

GluR1

UniProt

P19490

NCBI Gene ID

50592

RefSeq (Protein):NP_113796