GLO1 Antibody (N-term)

SKU: orb1929915

Description

GLO1 Antibody (N-term)

Research Area

Cancer Biology, Cardiovascular Research, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 20778 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Flow cytometric analysis of widr cells using GLO1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of GLO1 Antibody (N-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human brain tissue reacted with GLO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of GLO1 antibody (N-term) in HL60 cell line lysates (35 ug/lane). GLO1 (arrow) was detected using the purified Pab.

Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.

All lanes: Anti-GLO1 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q04760