GLI1 Antibody

• Catalog Number: orb1259840
• Category: Antibodies
• Description: GLI1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human GLI1 (NP_001153517.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 130kDa/160kDa
• Target: GLI1
• UniProt ID: P08151
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: GLI1, GLI
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using GLI1 antibody (orb1259840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat testis using GLI1 antibody (orb1259840) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using GLI1 antibody (orb1259840) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using GLI1 antibody (orb1259840) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using GLI1 antibody (orb1259840) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody (orb1259840) at dilution of 1:100. Blue: DAPI for nuclear staining.