GJA1 Antibody

SKU: orb394902

Description

Rabbit polyclonal antibody to Connexin-43.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IHC, IP, WB
• Dilution range: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: gap junction protein, alpha 1, 43kDa
• Target: GJA1
• Molecular Weight: 47 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Disclaimer: For research use only

Alternative Names

Connexin-43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using orb394902 at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-Connexin-43 (IP: orb394902, 4 ug; Detection: orb394902 1:1000) with mouse heart tissue lysate 3200 ug.

HeLa cells were subjected to SDS PAGE followed by western blot with orb394902 (GJA1 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded mouse heart using orb394902 (GJA1 antibody) at dilution of 1:100.

Quick Database Links

Gene Symbol

GJA1

UniProt

P17302