GJA1 Antibody

• Catalog Number: orb394902
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Connexin-43.
• Target: GJA1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Immunogen: gap junction protein, alpha 1, 43kDa
• UniProt ID: P17302
• MW: 47 kDa
• Tested applications: ELISA, FC, IHC, IP, WB
• Dilution range: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Connexin-43, CX43, DFNB38, Gap junction alpha 1 pr
• Note: For research use only

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using orb394902 at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-Connexin-43 (IP: orb394902, 4 ug; Detection: orb394902 1:1000) with mouse heart tissue lysate 3200 ug.

HeLa cells were subjected to SDS PAGE followed by western blot with orb394902 (GJA1 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded mouse heart using orb394902 (GJA1 antibody) at dilution of 1:100.