GITR Antibody / TNFRSF18

• Catalog Number: orb749934
• Category: Antibodies
• Description: GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF-receptor superfamily, also known as TNFRSF18. It is expressed at low levels on resting T lymphocytes and at high levels on CD25+ CD4+ Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-kB activation, and abrogate the inhibitory function of CD25+ CD4+ Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: DTA-1
• Tested applications: FACS, IF
• Reactivity: Mouse
• Isotype: Rat IgG2b, lambda
• Immunogen: Mouse CD25+ CD4+ T cells were used as the immunogen for the GITR antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This GITR antibody is available for research use only.
• UniProt ID: O35714
• Storage: Store the GITR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the GITR antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.