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GIP Antibody

SKU: orb1257149

Description

GIP Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-153 of human GIP (NP_004114.1).
TargetGIP
Molecular WeightObserved: 17kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

gastric inhibitory polypeptide, glucose-dependent insulinotropic polypeptide, incretin hormone

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GIP Antibody

Western blot analysis of extracts of various cell lines, using GIP antibody (orb1257149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

GIP Antibody

Immunohistochemistry of paraffin-embedded rat kidney using GIP antibody (orb1257149) at dilution of 1:100 (40x lens).

GIP Antibody

Immunohistochemistry of paraffin-embedded human colon using GIP antibody (orb1257149) at dilution of 1:100 (40x lens).

GIP Antibody

Immunohistochemistry of paraffin-embedded human gastric cancer using GIP antibody (orb1257149) at dilution of 1:100 (40x lens).

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

GIP Antibody (orb1257149)

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100 μl
$ 830.00
DispatchUsually dispatched within 5-10 working days
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