GFP Antibody Peroxidase Conjugate

• Catalog Number: orb345333
• Category: Antibodies
• Description: GFP antibody (Peroxidase)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9F9.F9
• Tested applications: ELISA, IHC, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:80,000, IHC: 1:500 - 1:2,000, WB: 1:2,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: GFP Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
• Conjugation: HRP
• UniProt ID: P42212
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Thimerosal
• Alternative names: mouse anti-GFP antibody HRP conjugation, peroxidas
• Note: For research use only
• Application notes: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody was tested by western blot and ELISA. It can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western Blot of Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate. Lane 1: 50 ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate (p/n orb345333) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (p/n orb348540, diluted to 1X) 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.

Western Blot of Anti-GFP Monoclonal Antibody Peroxidase Conjugate. Lane 1: Super Signal Molecular Weight Marker. Lane 2: 50 ng of GFP. Primary antibody: Mouse Anti-GFP Peroxidase Conjugated Antibody (p/n orb345333) at 1:1000 overnight at 2-8°C. Secondary antibody: none. Block: BlockOut - Universal Blocking Buffer (p/n orb348644). Predicted/Observed size: 28kDa for GFP. Other band(s): none.