GFP Antibody Fluorescein Conjugated

• Catalog Number: orb345307
• Category: Antibodies
• Description: GFP antibody (FITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FLISA, IF, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: >1:20,000, IF: 1:500 - 1:2,500, WB: >1:10,000
• Form/Appearance: Lyophilized
• Purity: GFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins.
• Conjugation: FITC
• UniProt ID: P42212
• Storage: Store anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-GFP Antibody fluorescein Conjugation, FI
• Note: For research use only
• Application notes: Anti-GFP Fluorescein Conjugated Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Fluorescein conjugated Goat anti-GFP. Antigen: GFP Load: Five point 3-fold serial dilution starting at 100 ng (Dot 1). Primary antibody: None. Secondary Antibody: GFP Antibody Fluorescein Conjugated Pre-absorbed (p/n orb345307) at 1:1000 for 1hr at RT. Blocking: Blocking buffer for Fluorescent Blotting (p/n orb348637) Imaged with FITC filter.

Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody. Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.