GFP Antibody Biotin Conjugated

• Catalog Number: orb345877
• Category: Antibodies
• Description: GFP antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Biotin
• Reactivity: Other
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins.
• Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• UniProt ID: P42212
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, IF: 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Biotin Conjugated GFP Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
• Antibody Type: Primary Antibody
• Storage: Store Anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit anti-GFP antibody biotin conjugation, bioti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immuno-Fluorescence of Biotin Mouse anti-GFP antibody. Biotin mouse anti GFP used 1:5000.

Western Blot of Peroxidase conjugated Goat anti-Rabbit IgG antibody. Lane 1: 50 ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP Antibody Biotin Conjugated secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 45 min at RT. HRP Streptavidin (p/n orb348767) was used at 1:40000 in orb348637 for 30 min at 20°C. Block: 5% Blotto (p/n orb348624) 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.