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Catalog Number | orb1257476 |
---|---|
Category | Antibodies |
Description | GFER Antibody |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 81-205 of human GFER (NP_005253.3). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 23kDa |
Target | GFER |
UniProt ID | P55789 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | FAD-linked sulfhydryl oxidase ALR, Augmenter of li Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using GFER antibody (orb1257476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse stomach using GFER antibody (orb1257476) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using GFER antibody (orb1257476) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using GFER antibody (orb1257476) at dilution of 1:100 (40x lens).
ELISA, IHC, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |