GFAP R416WT Antibody: HRP

• Catalog Number: orb150545
• Category: Antibodies
• Description: Mouse monoclonal to GFAP R416WT (HRP). The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N206B/9 (Formerly sold as S206B-9)
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. > 50% identity with other proteins (Vimentin, Desmin and Peripherin).
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: HRP
• MW: 50kDa
• Target: GFAP
• Entrez: 2670
• UniProt ID: P14136
• NCBI: NP_001124491.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: Glial fibrillary acidic protein antibody, Intermed
• Note: For research use only
• Application notes: 1 µg/ml of SMC-442 was sufficient for detection of GFAP R416WT in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP R416WT Monoclonal Antibody, Clone N206B/9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GFAP R416WT Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GFAP R416WT Antibody (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone N206B/9. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody at 1:250.