GFAP Antibody / Glial Fibrillary Acidic Protein

• Catalog Number: orb1151443
• Category: Antibodies
• Description: Mouse monoclonal antibody to GFAP
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GFAP/4450
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human GFAP protein was used as the immunogen for the Glial Fibrillary Acidic Protein antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Glial Fibrillary Acidic Protein antibody is available for research use only.
• UniProt ID: P14136
• Storage: Aliquot the Glial Fibrillary Acidic Protein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cerebellum tissue with Glial Fibrillary Acidic Protein antibody (clone GFAP/4450). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebellum tissue with Glial Fibrillary Acidic Protein antibody (clone GFAP/4450). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebellum tissue with Glial Fibrillary Acidic Protein antibody (clone GFAP/4450). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Glial Fibrillary Acidic Protein antibody (clone GFAP/4450) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Glial Fibrillary Acidic Protein antibody (clone GFAP/4450). These results demonstrate the foremost specificity of the GFAP/4450 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.