General Reverse Triiodothyronine (rT3) ELISA Kit

SKU: orb781735

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Reverse Triiodothyronine(rT3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Reverse Triiodothyronine(rT3).

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 6000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: All
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 93.75-6000 pg/mL
• Sensitivity: 27.68 pg/mL
• Concentration: 6000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

RT3 ELISA Kit, r-T3 ELISA Kit, Reverse T3 ELISA Kit, (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid ELISA Kit, 3,3',5'-Triiodo-L-thyronine ELISA Kit