General Reverse Triiodothyronine (rT3) ELISA Kit

SKU: orb1806533

Description

General Reverse Triiodothyronine (rT3) ELISA Kit

Images & Validation

• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Reverse Triiodothyronine(rT3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Reverse Triiodothyronine(rT3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reverse Triiodothyronine(rT3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: All
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 93.75-6000pg/mL
• Sensitivity: 27.55 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine

Quick Database Links

Gene Symbol

rT3