General Reverse Triiodothyronine (rT3) ELISA Kit

• Catalog Number: orb1806533
• Category: Assays and Kits
• Description: General Reverse Triiodothyronine (rT3) ELISA Kit
• Reactivity: All
• Range: 93.75-6000 pg/mL
• Assay Time: 1.5H
• Target: rT3
• Sensitivity: 27.55 pg/mL
• Alternative names: RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydrox
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Reverse Triiodothyronine(rT3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Reverse Triiodothyronine(rT3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reverse Triiodothyronine(rT3) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, Plasma and Other biological fluids
• Expiration Date: Please enquire.