General Pregnenolone (PREG) ELISA Kit

• Catalog Number: orb2307999
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PREG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PREG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PREG in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: All
• Range: 15.63-1000 pg/mL
• Assay Time: 2.5h
• Concentration: 1000 pg/mL
• Sensitivity: 5.88 pg/mL
• Alternative names: Pregnenolone
• Note: For research use only
• Application notes: standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PREG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PREG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PREG in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma and other biological fluids
• Expiration Date: Please enquire.