You have no items in your shopping cart.
Cart summary
| Catalog Number | orb1806503 |
|---|---|
| Category | Assays and Kits |
| Description | General Pregnenolone (PREG) ELISA Kit |
| Reactivity | All |
| Range | 0.78-50 ng/mL |
| Assay Time | 1.5H |
| Target | PREG |
| Sensitivity | 0.47 ng/mL |
| Alternative names | Pregnenolone Read more... |
| Note | For research use only |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pregnenolone(PREG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pregnenolone(PREG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pregnenolone(PREG) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Sample Types | Serum, Plasma and Other biological fluids |
| Expiration Date | Please enquire. |
