General Androsterone (ADT) ELISA Kit

SKU: orb781763

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Androsterone(ADT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Androsterone(ADT).

Research Area

Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 90000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ADT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ADT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: All
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 1406.25-90000 pg/mL
• Sensitivity: 147 pg/mL
• Concentration: 90000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

17-Ketosteroid ELISA Kit, 17-KS ELISA Kit, 3a-Hydroxy-17-Androstanone ELISA Kit