General Androsterone (ADT) ELISA Kit

SKU: orb1806536

Description

General Androsterone (ADT) ELISA Kit

Images & Validation

• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Androsterone(ADT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Androsterone(ADT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Androsterone(ADT) in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: All
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 1406.25-90000pg/mL
• Sensitivity: 144 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone

Quick Database Links

Gene Symbol

ADT