General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

• Catalog Number: orb1806502
• Category: Assays and Kits
• Description: General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit
• Reactivity: All
• Range: 1.56-100 ng/mL
• Assay Time: 1.5H
• Target: 8-OHdG
• Sensitivity: 0.94 ng/mL
• Alternative names: 7,8-Dihydro-8-oxo-2'-deoxyguanosine; 7,8-Dihydro-8
• Note: For research use only
• Application notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-OHdG. During the reaction, 8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-OHdG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-OHdG in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Sample Types: Serum,Plasma
• Expiration Date: Please enquire.