Gemin 4 Polyclonal Antibody

SKU: orb1420499

Description

Rabbit polyclonal antibody to Gemin 4.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Disclaimer: For research use only

Alternative Names

Gem-associated protein 4, Gemin-4, Component of gems 4, p97, GEMIN4

Sample: 293T (Human) Cell Lysate at 30 ug. Primary: Anti- Gemin 4 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 120 kD. Observed band size: 120 kD.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Gemin 4) Polyclonal Antibody, Unconjugated at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Quick Database Links

UniProt

P57678