Gelsolin Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb704177
• Category: Antibodies
• Description: Gelsolin Recombinant Rabbit Monoclonal Antibody
• Target: GSN
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human Gelsolin protein
• UniProt ID: P06396
• MW: 80 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
• Antibody Type: Recombinant Antibody
• Clone Number: 2G6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Actin depolymerizing factor; ADF; AGEL; Amyloidosi
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Human plasma, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 81 kDa, Observed MW: 81 kDa.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gelsolin) Monoclonal Antibody, Unconjugated (orb704177) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Lane 3: Hela (Human) Cell Lysate at 30 ug, Lane 4: U251 (Human) Cell Lysate at 30 ug, Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 6: Siha (Human) Cell Lysate at 30 ug, Primary: Anti-Gelsolin (orb704177) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kD, Observed band size: 90 kD.

Sample: Lane 1: Plasma (Mouse) at 20 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Lane 4: Hela (Human) Cell Lysate at 30 ug, Lane 5: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-Gelsolin (orb704177) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kD, Observed band size: 90 kD.