GDNF Antibody: Biotin

• Catalog Number: orb2652577
• Category: Antibodies
• Description: Rabbit Anti-Human GDNF Polyclonal
• Target: GDNF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Biotin
• Reactivity: Human, Mouse, Rat
• Concentration: 1mg/mL
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Immunogen: Recombinant Human GDNF Protein, Full length (AA78-211)
• UniProt ID: P39905
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); optimal dilutions for assays should be determined by the user.
• Application notes: 1 µg/mL of SPC-810 was sufficient for detection of GDNF in 15 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Glial cell line-derived neurotrophic factor Antibo
• Note: For research use only

Western blot analysis with anti-GDNF polyclonal antibody showing detection of human recombinant GDNF protein. This recombinant protein has a predicted molecular weight of 15.2 kDa. Block: 5% skim milk. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:5000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min in RT. Exposed 9.122 seconds.

Western blot analysis with anti-GDNF polyclonal antibody showing detection of GDNF protein in rat brain lysate (prepared in-house). Rat GNDF protein with signal and pro-peptide segments has a predicted molecular weight of 23.6 kDa (UniProt Q07731). Block: 5% skim milk. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min in RT. Exposed 7.299 seconds. NOTE secondary antibody shows some non-specific binding in the control, but not to the GNDF protein.