GDF15 Antibody Peroxidase Conjugated

• Catalog Number: orb345861
• Category: Antibodies
• Description: NAG-1 antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody specifically reacts with a H variant sequence of human NAG-1 protein from human tissues. A BLAST analysis was used to suggest partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.
• Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
• UniProt ID: Q99988
• Tested applications: ELISA
• Dilution range: ELISA: User Optimized, WB: User Optimized
• Application notes: This affinity purified antibody is tested for use in ELISA and suitable for western blotting assays. This reagent is particularly useful to differentiate polymorphic forms of NAG-1 protein present in human serum samples. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user.
• Antibody Type: Primary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit anti-NAG1 antibody peroxidase conjugation,
• Note: For research use only
• NCBI: Q99988.3
• Expiration Date: 12 months from date of receipt.

In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies (see Related Products below): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/ml. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µl per well at 1 µg/ml. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.