GDF15 Antibody

• Catalog Number: orb1537796
• Category: Antibodies
• Description: GDF15 Antibody
• Target: GDF15
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-QKTDTGVSLQTYDD, from the C-Terminus of protein sequence according to NP_004855.2.
• Tested applications: ELISA, FC, IHC, IHC-P, WB
• Dilution range: Flo (10 µg/ml), IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:2000), WB (0.1 - 0.3 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B2413 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2413 was determined to be 5 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GDF15, MIC1, MIC-1, NAG-1, NRG-1, NSAID-regulated
• Note: For research use only

Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Goat Anti-GDF15 Antibody (0.3 µg/ml) staining of Human Testes (A) and negative control Skin (B) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescencence.

Goat Anti-GDF15 Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.