GDF15 Antibody

• Catalog Number: orb1247958
• Category: Antibodies
• Description: GDF15 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QKTDTGVSLQTYDD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 30-35 kDa band observed in Human Testis lysates (calculated MW of 34.1kDa according to NP_004855.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Skin lysate. In transfected HEK293 transiently expressing GDF15 a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293. Preliminary testing was unsuccessful on Mouse for this particular batch. IHC: Paraffin embedded Human Prostate and Placenta. Recommended concentration: 5µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GDF15
• UniProt ID: Q99988
• NCBI: NP_004855.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GDF15, PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 30-35 kDa band observed in Human Testis lysates (calculated MW of 34.1kDa according to NP_004855.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Skin lysate. In transfected HEK293 transiently expressing GDF15 a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293. Preliminary testing was unsuccessful on Mouse for this particular batch. IHC: Paraffin embedded Human Prostate and Placenta. Recommended concentration: 5µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247958 (0.3 μg/ml) staining of Human Testes (A) and negative control Skin (B) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

HEK293 overexpressing GDF15 (RC201295) and probed with orb1247958 (mock transfection in first lane).

orb1247958 (5 μg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247958 (5 μg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247958 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.