GDF15 Antibody Biotin Conjugated

SKU: orb345179

Description

NAG-1 antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Reactivity: Human, Mouse
• Application Notes:
  This Protein-A purified, MBP-cross-adsorbed NAG-1 Biotin conjugated antibody has ben tested by Western blot of human and mouse NAG-1 protein and is suitable for ELISA. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 C-terminal specific antibodies. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user. Expect bands in western blots of approximately 14 kDa in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.
• Purity: This antibody reacts with endogenous NAG-1 protein from human tissues. A BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee based on 100% and 98% homology, respectively, and with macaque based on an 94% homology, and with mouse and rat based on 70% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

rabbit anti-NAG-1 biotin Conjugated Antibody, NAG1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor

Western Blot of Rabbit Anti-NAG-1 Biotin Conjugated Antibody. Lane 1: Rec. Human NAG1 (yeast expressed) reduced (0.1 µg). Lane 2: Opal Prestained Molecular Weight Marker (5 µl). Lane 3: Rec. Human NAG1 (yeast expressed) non-reduced (0.1 µg). Primary Antibody: Anti-NAG1 Biotin Conjugate at 1 µg/ml overnight at 2-8°C. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT. Blocking buffer: (p/n orb348637). Expect/Observed: ~14kDa.

Quick Database Links

UniProt

Q99988

RefSeq:Q99988.3