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Item 1 of 3
GCK Antibody (C-term)
Catalog Number: orb1928779
| Catalog Number | orb1928779 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB05339 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 52191 Da |
| Target | This GCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 403-433 amino acids from the C-terminal region of human GCK. |
| UniProt ID | P35557 |
| NCBI | NP_000153.1, NP_277042.1, NP_277043.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Glucokinase, Hexokinase type IV, HK IV, Hexokinase Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of hGCK-S418 in Jurkat cell line lysates (35 ug/lane). GCK (arrow) was detected using the purified Pab.
Western blot analysis of GCK (arrow) using rabbit polyclonal hGCK-S418. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCK gene.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
