GCDFP-15 Antibody / PIP / Prolactin-Induced Protein

• Catalog Number: orb386022
• Category: Antibodies
• Description: It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15), also called Prolactin Inducible Protein, or PIP. It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. PIP is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PIP/1571
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A human partial recombinant protein corresponding to amino acids 41-146 was used as the immunogen for the PIP antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This PIP antibody is available for research use only.
• UniProt ID: P12273
• Storage: Store the PIP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the PIP antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human breast carcinoma with PIP antibody (clone PIP/1571). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20.

Western blot testing of human 1) pancreas and 2) HepG2 lysate with PIP antibody (clone PIP/1571). Expected molecular weight ~15 kDa.

SDS-PAGE Analysis of Purified, BSA-Free PIP Antibody (clone PIP/1571). Confirmation of Integrity and Purity of the Antibody.