PIP Antibody / Prolactin Inducible Protein / GCDFP-15

SKU: orb386022

Description

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15), also called Prolactin Inducible Protein, or PIP. It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. PIP is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Titering of the PIP antibody may be required for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: PIP/1571
• Immunogen: A human partial recombinant protein corresponding to amino acids 41-146 was used as the immunogen for the PIP antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human breast carcinoma with PIP antibody (clone PIP/1571). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20.

Western blot testing of human 1) pancreas and 2) HepG2 lysate with PIP antibody (clone PIP/1571). Expected molecular weight ~15 kDa.

SDS-PAGE Analysis of Purified, BSA-Free PIP Antibody (clone PIP/1571). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P12273