Prolactin-Induced Protein Antibody / PIP / GCDFP-15

SKU: orb1822932

Description

It recognizes a protein of 15kDa, identified as Prolactin-Induced Protein (PIP), also called Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. Prolactin inducible protein is a prolactin and androgen-controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Prolactin-Induced Protein antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: PIP/7477
• Immunogen: A recombinant partial protein sequence (within amino acids 41-146) from the human protein was used as the immunogen for the Prolactin-Induced Protein antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human breast carcinoma tissue with Prolactin-Induced Protein antibody (clone PIP/7477). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Prolactin-Induced Protein antibody (clone PIP/7477) as confirmation of integrity and purity.

Quick Database Links

UniProt

P12273