GBX2 Antibody

• Catalog Number: orb1247456
• Category: Antibodies
• Description: GBX2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QGKDESKVEDDPK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in lysates of T-cell line Jurkat (calculated MW of 37.3kDa according to NP_001476.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH-3T3 cell line for this particular batch.Immunohistochemistry:In paraffin embedded Human Placenta shows nuclear staining of selected trophoblasts. Recommended concentration: 4-8ug/ml. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GBX2
• UniProt ID: P52951
• NCBI: NP_001476.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GBX2, gastrulation brain homeobox 2 , gastrulation
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in lysates of T-cell line Jurkat (calculated MW of 37.3kDa according to NP_001476.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH-3T3 cell line for this particular batch.Immunohistochemistry:In paraffin embedded Human Placenta shows nuclear staining of selected trophoblasts. Recommended concentration: 4-8ug/ml. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247456 (0.1 ug/ml) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247456 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247456 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.